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KMID : 0380219870200020185
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Journal of Biochemistry and Molecular Biology
1987 Volume.20 No. 2 p.185 ~ p.190
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2,3,5-Triiodobenzoic Acid as an Inhibitor of Ethylene Forming Enzyme in Mung Bean Hypocotyls
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Kim Soon-Young
Kang Bin-Goo
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Abstract
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Auxin-induced ethylene production in hypocotyl segments of etiolated mung bean seedlings was severely inhibited by 2,3,5-triiodobenzoic acid (TIBA), a potent inhibitor of auxin transport. However. the inhibition of ethylene biosynthesis by the transport inhibitor was found to be unrelated to an obvious impairment of the auxin transport system. The inhibitor also suppressed ethylene production from segments fed with 1-aminocyclopropane-1-carboxylic acid (ACC). the immediate precursor of ethylene. TIBA treatment also led to a substantial accumulation of ACC in the tissue incubated with either auxin or ACC. These findings suggest that TIBA inhibits ethylene production by specifically blocking the conversion of ACC to ethylene. The possibility of TIBA acting as a free radical scavenger was ruled out by the lack of TIBA effect on chemiluminescence in a luminol-based system in vitro. Results obtained from experiments with mercaptoethanol suggest that TIBA may act as a sulfhydryl inhibitor on "ethylene forming enzyme (EFE)", the enzyme catalyzing the formation of ethylene from the penultimate precursor, ACC.
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